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vector 1 c  (Addgene inc)


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    Addgene inc vector 1 c
    Vector 1 C, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector 1 c/product/Addgene inc
    Average 93 stars, based on 18 article reviews
    vector 1 c - by Bioz Stars, 2026-06
    93/100 stars

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    a Strep-tag pull-down assay demonstrating interaction of ICP8 with either full-length UL9 or UL9CTD. b GST pull-down assay analyzing binding between ICP8 and either UL9CTD or the C-tail. c Comparative ATPase activity of wild-type UL9 versus its functional mutants. ATPase activity assays were performed using the Malachite Green Phosphate Detection Kit. Data are presented as the mean with standard deviation for experiments performed in triplicate. Statistical significance was determined by a two-tailed Student’s t -test (*p < 0.05, **p < 0.01, *p < 0.001). d ICP8-mediated stimulation of UL9 helicase activity. Wild-type UL9 and two C-terminal truncation mutants (ΔC16 and ΔC8) were tested.

    Journal: bioRxiv

    Article Title: Structural basis of HSV-1 DNA replication origin-binding protein UL9

    doi: 10.1101/2025.10.31.685793

    Figure Lengend Snippet: a Strep-tag pull-down assay demonstrating interaction of ICP8 with either full-length UL9 or UL9CTD. b GST pull-down assay analyzing binding between ICP8 and either UL9CTD or the C-tail. c Comparative ATPase activity of wild-type UL9 versus its functional mutants. ATPase activity assays were performed using the Malachite Green Phosphate Detection Kit. Data are presented as the mean with standard deviation for experiments performed in triplicate. Statistical significance was determined by a two-tailed Student’s t -test (*p < 0.05, **p < 0.01, *p < 0.001). d ICP8-mediated stimulation of UL9 helicase activity. Wild-type UL9 and two C-terminal truncation mutants (ΔC16 and ΔC8) were tested.

    Article Snippet: UL9 CTD and its truncation mutants used for GST pull-down assays, along with two C-tail fragments, were cloned into the pGEX-6P-1 vector (GE Healthcare) to generate GST-tagged fusion proteins.

    Techniques: Strep-tag, Pull Down Assay, Binding Assay, Activity Assay, Functional Assay, Standard Deviation, Two Tailed Test